IUCN / SSC Cat Specialist Group - Digital Cat Library |
|
||||||||||
|
||||||||||
|
|
| Zou, Z.-T.; Uphyrkina, O.V.; Fomenko, P.; Luo, S.-J. | |
| The development and application of a multiplex short tandem repeat (STR) system for identifying subspecies, individuals and sex in tigers | |
| 2015 Ecosphere (4): 1-19 | |
Poaching and trans-boundary trafficking of tigers and body parts are threatening the world's last remaining wild tigers. Development of an efficient molecular genetic assay for tracing the origins of confiscated specimens will assist in law enforcement and wildlife forensics for this iconic flagship species. We developed a multiplex genotyping system "tigrisPlex" to simultaneously assess 22 short tandem repeat (STR, or microsatellite) loci and a gender-identifying SRY gene, all amplified in 4 reactions using as little as 1 ng of template DNA. With DNA samples used for between-run calibration, the system generates STR genotypes that are directly compatible with voucher tiger subspecies genetic profiles, hence making it possible to identify subspecies via bi-parentally inherited markers. We applied "tigrisPlex" to 12 confiscated specimens from Russia and identified 6 individuals (3 females and 3 males), each represented by duplicated samples and all designated as Amur tigers (_Panthera tigris altaica_) with high confidence. This STR multiplex system can serve as an effective and versatile approach for genetic profiling of both wild and captive tigers as well as confiscated tiger products, fulfilling various conservation needs for identifying the origins of tiger samples. |
|
 |
PDF files are only accessible to Friends of the Cat Group. Joining Friends of the Cat Group gives you unlimited access and downloads in the Cat SG Library for one year, and allows you to receive our newsletter Cat News (2 regular issues per year plus special issues). More information how to join here |
(c) IUCN/SSC Cat Specialist Group ( IUCN - The World Conservation Union) |