IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Zhang, F.; Jiang, Z.; Zeng, Y.; McCarthy, T. | |
| Development of primers to characterize the mitochondrial control region of the snow leopard | |
| 2007 Molecular Ecology Notes (7): 1196-1198 | |
The snow leopard (_Uncia uncia_) is a rare carnivore living above the snow line in central Asia. Using universal primers for the mitochondrial genome control region hypervariable region 1 (HVR1), we isolated a 411-bp fragment of HVR1 and then designed specific primers near each end of this sequence in the conserved regions. These primers were shown to yield good polymerase chain reaction products and to be species specific. Of the 12 snow leopards studied, there were 11 segregating sites and six haplotypes. An identification case of snow leopard carcass (confiscated by the police) proved the primers to be a useful tool for forensic diagnosis in field and population genetics studies. |
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