IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Barr, M.C.; Zou, L.; Long, F.; Hoose, W.A.; Avery, R.J. | |
| Proviral organization and sequence analysis of feline immunodeficiency virus isolated from a Pallas' cat | |
| 1997 Virology (228): 84-91 | |
The nucleotide sequence and genomic organization have been determined for a highly cytopathic feline immunodeficiency virus (FIV) isolated from a Pallas' cat. The 9747-bp provirus of this virus, FIV-Oma, has typical lentivirus organization with LTRs, _gag_, _pol_, and _env_ open reading frames (ORFs), putative _vif_ and _ref_ ORFs, and an ORF similar to ORF2/ORFA of domestic cat FIV isolates. Although the FIV-Oma provirus is 300 to 600 bp longer than other FIV proviruses, these additional bases are distributed throughout the genome. Phylogenetic analysis of a conserved region of the _pol_ gene suggests that FIV-Oma is more closely related to some of the puma and lion lentiviruses than it is to domestic cat FIV isolates; however, many regions of the genome exhibit extensive nucleotide sequence divergence. None of the eight molecular proviral clones isolated form a genomic library are infectious, but we have constructed an infectious, cytopathic clone of FIV-Oma from subcloned and PCR-amplified fragments of these proviral clones. This clone will be useful for identifying the genetic determinants of FIV-Oma's biological activities. |
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