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Pospischil, A.; Kaiser, C.; Hofmann-Lehmann, R.; Lutz, H.; Hilbe, M.; Vaughan, L.; Borel, N.
Evidence for _Chlamydia_ in wild mammals of the Serengeti
2012  Journal of Wildlife Diseases (48): 1074-1078

Only limited information is available on the presence of _Chlamydiaceae_ in wildlife, a deficit that is particularly acute concerning mammalian wildlife in Africa. In a retrospective analysis of organ material from an earlier study on wild mammals from the Seregenti National Park, 521 samples from 54 animals of 14 mammalian species were investigated. The presence of _Chlamydiaceae_ was analyzed using molecular methods and immunohistochemistry. Chlamydial DNA was detected by real-time polymerase chain reaction in formalin-fixed and paraffin-embedded tissues from large ruminants (African buffaloes, _Syncerus caffer_, n=4) and a large predator (spotted hyena, _Crocuta crocuta_, n=1). Microarray results revealed _Chlamydia abortus_ in all cases, confirmed by sequencing of selected samples, and a mixed infection with _Chlamydia abortus_ and _Chlamydia pneumoniae_ in an African buffalo. This is the first report of _Chlamydiaceae_ in African wildlife of the Serengeti area.

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