IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Dias, E.A. | |
| Avalia‡Æo do perfil de progestinas e metab¢litos de glicocortic¢ides em fezes de jaguatiricas submetidas ao desafio com ACTH | |
| 2006 Full Book | |
Two commercial kits, the ImmuChem Doubly Antibody Corticosterona 125I RIE from ICN Biomedicals and Coat-a-count Cortisol 125I RIE from DPC were used on fecal glucocorticoids measurement of ocelots (_Leopardus pardalis_) submitted to ACTH challenge aiming to compare the performance of both kits. In another way progestins from the same animals submitted to ACTH challenge were measured aiming to find progesterone from extra gonadal sources. Two different fecal metabolites extraction protocols were tested as well. The ICN commercial kit had better results on glucocorticoids metabolites measurement. Both extraction protocols had similar performance and this data indicates that any both can be used for this purpose. There are strong evidences of adrenal secretion of progesterone after ACTH challenge. |
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