IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Guiao-Leite, F.L. | |
| Avalia‡Æo da eficiˆncia da eletroforese capilar como t‚cnica anal¡tica na prospec‡Æo de metab¢litos de ester¢ides em extratos fecais de on‡a-pintada | |
| 2006 Full Book | |
The objective of this work was to develop a protocol of hormonal extraction from jaguar feces (_Panthera onca_) and validate a methodology of analysis of these extracts with capillary electrophoresis, verifying the analytical efficiency of this technique in the study of the steroids metabolites in feces, with the objective of future use of this methodology in the routine of the endocrinology laboratories. Seven treatments were tested for the samples: four extraction protocols in different solvents, one of acid hydrolysis and two extraction protocols in solid phase (SPE). The extraction protocol in acetonitrila was satisfactory as pre-treatment of the samples and ally to SPE C-18, it presented good results with respect to the sensibility, precision, recovery and time of analysis. Based on the obtained results, the capillary electrophoresis can be considered a great alternative for the research of metabolites of hormones, it could also be used in routine analyses in the endocrinology laboratories, mainly in those that are devoted to the non-invasive evaluation methodologies of the reproductive function in wild animals. |
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