IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Pushett, D. | |
| Assisted reproduction and subsequent embryo development to form embryos for transfer and cryobanking in endangered cats using the domestic cat as a model | |
| 2000 Full Book | |
All 36 non-domestic cat species are considered either threatened or endangered. Employing assisted reproductive techniques may be the only way of ensuring long term survival of these species. Studies in assisted reproduction utilizing endangered cat species are difficult, due to low numbers and the limited availability of reproductive material. The domestic cat can be a model for establishing assisted reproductive techniques for application to rare and endangered cat species. A system for successful production of an cryopreservation of domestic cat embryos from gametes retrieved following castration was investigated. Techniques and protocols for processing and maturing oocytes and spermatozoa and for freezing and thawing spermatozoa were established. Systems for _in vitro _fertilization (IVF), intracytoplasmic sperm injection (ICSI), embryo culture and embryo cryopreservation were developed. Embryos derived from IVF and ICSI were cryopreserved, warmed and transferred to recipient queens to determine the efficacy of the techniques. A successful system has now been developed for _in vitro _production of domestic cat embryos with gametes retrieved from reproductive organs following castration. The system includes _in vitro _oocyte maturation, sperm cryopreservation, IVF and ICSI, _in vitro _embryo culture and embryo cryopreservation. The techniques and protocols developed throughout this research program should now be applied to rare and endangered species as the opportunity arises and may result in the preventing their extinction. |
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