IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Salame-Mendez, A.; Andrade-Herrera, M.; Zamora-Torres, L.; Serrano, H.; Soto-Mendoza, S.; Castro-Campillo, A.; Ramirez-Pulido, J.; Haro-Castellanos, J. | |
| M‚todo optimizado para evaluar cidos biliares de muestras fecales secas o preservadas en etanol como herramienta para identificar carn¡voros silvestres | |
| 2012 Acta Zoologica Mexicana (28): 305-320 | |
We optimize several aspects involved in the method for a reliable, more simple, and easy obtaining of bile acids (AB) and cholesterol (Co) from the feces of wild carnivores, including: selection of the optimal developer; adaptation of the mobile phase in a first chromatographic evaluation; optimization of separation through a second chromatographic evaluation with another mobile phase; the use of minimal amounts of both fecal sample (mg) or the ethanolic solution (L) in which the former were preserved. The new procedure is simpler and easily reproducible, and thus it turns out to be an analytic, non-invasive, and feasible tool for the identification of wild carnivores by means of the AB within their feces. In order to probe the method, we analyzed feces from _Puma concolor_, _Panthera onca_, _Canis lupus baileyi_, and _Canis latrans _maintained in captivity, which are also described and, therefore, we also present for the first time, the profile of AB for the Mexican wolf. |
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