IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Verma, S.K.; Minicucci, L.; Murphy, D.; Carstensen, M.; Humpal, C.; Wolf, P.; Calero-Bernal, R.; Cerqueira-Cezar, C.K.; Kwok, O.C.H.; Su, C.; Hill, D.; Dubey, J.P. | |
| Antibody detection and molecular characterization of _Toxoplasma gondii_ from bobcats, domestic cats, and wildlife from Minnesota, USA | |
| 2016 Journal of Eukaryotic Microbiol (63): 567-571 | |
Little is known of the epidemiology of toxoplasmosis in Minnesota. Here, we evaluated _Toxoplasma gondii_ infection in 50 wild bobcats (_Lynx rufus_) and 75 other animals on/near 10 cattle farms. Antibodies to _T. gondii_ were assayed in serum samples or tissue fluids by the modified agglutination test (MAT, cut-off 1:25). Twenty nine of 50 bobcats and 15 of 41 wildlife trapped on the vicinity of 10 farms and nine of 16 adult domestic cats (_Felis catus_) and six of 14 domestic dogs resident on farms were seropositive. _Toxoplasma gondii_ oocysts were not found in feces of any felid. Tissues of all seropositive wild animals trapped on the farm were bioassayed in mice and viable _T. gondii_ was isolated from two badgers (_Taxidea taxus_), two raccoons (_Procyon lotor_), one coyote (_Canis latrans_), and one opossum (_Didelphis virginiana_). All six _T. gondii_ isolates were further propagated in cell culture. Multi-locus PCR-RFLP genotyping using 10 markers (SAG1, SAG2 (50-30SAG2, and alt.SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico), and DNA from cell culture derived tachyzoites revealed three genotypes; #5 ToxoDataBase (1 coyote, 1 raccoon), #1 (1 badger, 1 raccoon, 1 opossum), and #2 (1 badger). This is the first report of _T. gondii_ prevalence in domestic cats and in bobcats from Minnesota, and the first isolation of viable _T. gondii_ from badger. |
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