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| Wei, L.; Wu, X.; Jiang, Z. | |
| The complete mitochondrial genome structure of snow leopard | |
| 2009 Molecular Biology Reports (36): 871-878 | |
The complete mitochondrial genome (mtDNA) of snow leopard _Panthera uncia_ was obtained by using the polymerase chain reaction (PCR) technique based on the PCR fragments of 30 primers we designed. The entire mtDNA sequence was 16 773 base pairs (bp) in length, and the base composition was: A-5,357 bp (31.9%); C-4,444 bp (26.5%); G-2,428 bp (14.5%); T-4,544 bp (27.1%). The structural characteristics [0] of the _P. uncia_ mitochondrial genome were highly similar to these of _Felis catus_, _Acinonyx jubatus_, _Neofelis nebulosa_ and other mammals. However, we found several distinctive features of the mitochondrial genome of Panthera unica. First, the termination codon of COIII was TAA, which differed from those of _F. catus_, _A. jubatus_ and _N. nebulosa_. Second, tRNASer (AGY), which lacked the ''DHU'' arm, could not be folded into the typical cloverleaf-shaped structure. Third, in the control region, a long repetitive sequence in RS-2 (32 bp) region was found with 2 repeats while one short repetitive segment (9 bp) was found with 15 repeats in the RS-3 region. We performed phylogenetic analysis based on a 3 816 bp concatenated sequence of 12S rRNA, 16S rRNA,ND2, ND4, ND5, Cyt b and ATP8 for _P. uncia_ and other related species, the result indicated that _P. uncia_ and _P. leo_ were the sister species, which was different from the previous findings. |
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