IUCN/SSC Cat Specialist Group Library

IUCN / SSC Cat Specialist Group - Digital Cat Library

View printer friendly
Alisha, A.; Minakshi, P.; Ranjan, K.; Prasad, G.
Comparison of D-Loop gene for subspecies confirmation of tigers
2014 Advances in Animal and Veterinary Sciences (2): 116-119
Tiger (_Panthera tigris_), the biggest cat species and the top predator has fallen into endangered category. In the present study, we confirmed the subspecies of tiger with help of DNA based methods. The mitochondrial control region gene (D-loop) sequence was utilized for this purpose. DNA was extracted from tiger blood sample originally obtained from mini zoo of Rohtak, Haryana. The D-loop sequence was amplified and submitted to GenBank with accession number HM362438. Based on D-loop sequence identity and phylogenetic analysis _Panthera tigris tigris_ (Indian tiger) was differentiated from other _Panthera tigris_ subspecies (_Panthera tigris amoyensis_, _Panthera tigris corbetti_, _Panthera tigris sumatrae_ and _Panthera tigris altaica_) from different parts of the world. The present report also describes an easier, quicker and non hazardous protocol for the isolation of genomic DNA from tiger blood.
	PDF files are only accessible to Friends of the Cat Group. Joining Friends of the Cat Group gives you unlimited access and downloads in the Cat SG Library for one year, and allows you to receive our newsletter Cat News (2 regular issues per year plus special issues). More information how to join here