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Dresser, B.L.; Sehlhorst, C.S.; Keller, G.L.; Kramer, L.W.; Reece, B.
Artificial insemination and embryo transfer in the Felidae
1987  Book Chapter

The potential for techniques such as artificial insemination becomes apparent when the risks of having to transport large, dangerous or rare animals are considered. Likewise, embryo transfer. a technique in which a fertilized ovum is transferred to a recipient mother for the tenure of gestation and ultimate birth, has great potential for ensuring the continuity of particular genetic lines and bringing isolated animals into a breeding program. Through freezing, this technique also allows for the preservation of genes for future use in different populations. Aged females, no longer contributors to a breeding group, may still remain a valuable source of eggs or embryos for transplantation. Inferior females, whose genetic qualities are undesirable, are still able to nurture a transplanted embryo through gestation and function in a surrogate male. Methods also need to be developed whereby the number of offspring from an endangered species can increase by the use of a more common member of another closely related species through the determination of surrogate mothers (interspecies embryo transfer). Since conservation of endangered species is no longer a prerogative but a responsibility, research must be designed to insure the propagation and survival of wildlife. Since domestic cats and most exotic cats (Wildt et al. 1980) are induced ovulators, induction of ovulation must be achieved by a copulation stimulus or through an exogenous source of luteinizing hormone. Thus, ovulation may be induced by mating with a vasectomized male or by injection of human chorionic gonadotropin (HCG) on days 1 and 2 of estrus (Platz et al. 1978). Ovulation occurs within 25-26.6 hours of an intramuscular or intravenous injection of HCG (Sojka et al. 1970).

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