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Dresser, B.L.; Gelwicks, E.J.; Wachs, K.B.; Keller, G.L.
First Successful Transfer of Cryopreserved Feline (Felis catus) Embryos Resultiong in Live Offspring
1988 Journal of Experimental Zoology (246): 180-186
Sexually mature domestic cats were hormonally stimulated to induce super-ovulation; embryo recovery was accomplished by laparotomy. Embryos were frozen by conventional embryo freezing methods used in the domestic cattle embrvo transfer industry. Thawing was achieved in a 28øC or 37øC waterbath or in ambient air. Overnight culture of the frozen-thawed embryos in a supplemented Nutrient Mixture F-10 (Ham's) or Earle's Balanced Salt Solution with 20% heat treated newborn calf serum resulted in five successful term litters from recipient queens. Embryo recipients who became pregnant had been treated with a subcutaneous injection of follicle-stimulating hormone (FSH-P) once every 24 hr for 6 days in the amount of 0.2 mg/day for the first 5 days and 0.1 mg on the sixth day, followed by two intramuscular 750 IU injections of human chorionic gonadotropin 24 hr apart, beginning on the same day as the sixth injection of FSH-P.
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