IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Gelwicks, E.J.; Pope, C.E.; Turner, J.L.; Keller, G.L.; Dresser, B.L. | |
| An evaluation of meiotic stages and polyspermy in uncleaved oocytes following in vitro fertilization in domestic cats | |
| 1990 Theriogenology (33): 228 | |
Typically, the presence of expanded corona radiata and cumulus cells surrounding an oocyte is indicative of its meiotic maturity, i.e. metaphase II (MII). During our studies on in vitro fertilization (IVF) in domestic cats, the cleavage rate of oocytes that were in an expanded cumulus mass at recovery has seldom reached 100% and usually ranged from 50% to 70%. In an effort to determine why these presumably MII stage oocytes did not develop in vitro, we evaluated their stage of meiotic maturation following fixation and staining at 48 to 96 h post-insemination. Follicular oocytes were aspirated via laparotomy from 31 domestic cats following treatment with an average total dose of 3.1 mg of follicle stimulating hormone (FSH-P, Schering) in a decreasing daily dose for four days. Human chorionic gonadotropin (Hcg, 100 IU) was given on day 5. A total of 939 oocytes (X=30.3) were recovered at 25 to 27 h post-Hcg, OF WHICH 846 (90%) were surrounded by an expanded cumulus mass. Sixty-four of these were excluded either due to a degenerate appearance or loss during the fertilization process. |
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