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Williamson, J.E.; Huebinger, R.M.; Sommer, J.A.; Louis, E.E.; Barber, R.C. | |
Development and cross-species amplification of 18 microsatellite markers in the Sumatran tiger (Panthera tigris sumatrae) | |
2002 Molecular Ecology Notes (2): 110-112 | |
Eighteen polymorphic microsatellite loci from the highly endangered Sumatran tiger (_Panthera tigris sumatrae_) were isolated and characterized. Upon polymerase chain reaction amplification, 16 of these markers produced a single, sharp band in all three tiger and 10 non-tiger felid species examined. Of the two remaining loci, 6HDZ057 and 6HDZ635 failed to amplify genomic DNA from puma (_Felis concolor_) and cheetah (_Acinonyx jubatus_), respectively. The amplification of these markers across four genera is an indication of their usefulness for population genetics studies and conservation work in a wide range of felid species. |
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(c) IUCN/SSC Cat Specialist Group ( IUCN - The World Conservation Union) |