IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Cossios, D.; Angers, B. | |
| Identification of Andean felid feces using PCR-RFLP | |
| 2006 Mastozoologia Neotropical (13): 239-244 | |
DNA methods on scat use intestinal cells of the animal that are incorporated into feces. Molecular scatology has been demonstrated to be an efficient method to identify carnivore species in a large number of publications, including felids (e.g. Farrel et al., 2000; Ernest et al., 2000; Palomares et al., 2002; Wan et al., 2003; Zuercher et al., 2003). However, no protocol specific to Andean felids has been published. Several projects focussing on Andean cat (_Oreailurus jacobita_), one of the rarest felids of the world and the most threatened of South America, are in progress (Villalba et al., 2004). Therefore the development of such a method is highly relevant. The aim of this work was to develop a PCRbased method for distinguishing between scats of Andean felids, namely Andean cat (_Oreailurus jacobita_), pampas cat (_Lynchailurus colocolo_) - the most common felid in the Andes-, Geoffroy's cat (_Oncifelis geoffroyi_), puma (_Puma concolor_), as well as other sympatric carnivores such as culpeo fox (_Pseudalopex culpaeus_), domestic cat (_Felis catus_), and domestic dog (_Canis familiaris_). A portion of the mitochondrial genome was PCR-amplified and RFLP patterns were used to distinguish felid species from one another and from other carnivore species. |
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