IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Fonseca-Salamanca, F.; Nogal-Ruiz, J.J.; Benito, C.; Camacho, M.V.; Martinez-Fern ndez, A.R. | |
| Molecular characterization of _Trichinella_ genotypes by inter-simple sequence repeat polymerase chain reaction (ISSR-PCR) | |
| 2006 Journal of Parasitology (92): 606-610 | |
A bulk analysis of inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) provides a quick, reliable, and highly informative system for DNA banding patterns that permit species identification. The present study evaluates the applicability of this system to Trichinella species identification. After a single amplification carried out on a single larva with the primer 816([CA]nRY) under high stringency conditions, which provide high reproducibility, we were able to identify by consistent banding patterns 5 sibling species: Trichinella spiralis (ISS48), 2 Trichinella britovi isolates (ISS11 and ISS86), Trichinella murrelli (ISS35), Trichinella nativa (ISS71), Trichinella nelsoni (ISS29); 3 additional Trichinella genotypes: T8 (ISS149), T9 (ISS408 and ISS409), and T6 (ISS34); and the nonencapsulated species Trichinella pseudospiralis (ISS13). Moreover, 33 new Trichinella isolates from 2 zoogeographical regions were unequivocally identified. All Trichinella isolates have shown an identical pattern with those produced by the reference strain. According to these data, we have demonstrated that ISSRPCR is a robust technique that emerges as a useful new application for the molecular identification of Trichinella isolates in epidemiological studies. |
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