IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Dubey, J.P.; Quirk, T.; Pitt, J.A.; Sundar, N.; Velmurugan, G.V.; Kwok, O.C.H.; Leclair, D.; Hills, R.; Su, C. | |
| Isolation and genetic characterization of toxoplasma gondii from raccoons (_Procyn rocyon lotor_), cats (_Felis domesticus_), striped skunk (_Mephitis mephitis_), black bear (_Ursus americanus_), and cougar (_Puma concolor_) from Canada | |
| 2008 Journal of Parasitology (94): 42-45 | |
Viable Toxoplasma gondii was isolated by bioassay in mice from tissues of 2 feral cats (_Felis domesticus_), 2 raccoons (_Procyon lotor_), a skunk (_Mephitis mephitis_) trapped in remote locations in Manitoba, Canada, and a black bear (_Ursus americanus_) from Kuujjuaq, northern Quebec, Canada. Genotyping of these T. gondii isolates using polymorphisms at 10 nuclear markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and an apicoplast marker Apico revealed 4 genotypes. None of the isolates was clonal archetypal Types I, II, and III found in the United States. These results are in contrast with the Type II genotype that is widespread in domestic animals and humans throughout the United States and Europe. This is the first genotyping of T. gondii isolates from this part of North America. |
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