IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Fonseca-Salamanca, F.; Nogal-Ruiz, J.J.; Garc¡a-S nchez, R.N.; Bolas-Fernandez, F.; µlamo, R.; G rate, T.; Mart¡nez-Fernandez, A.R.; Jim‚nez, S. | |
| Prevalence of _Trichinella_ spp. in North Spain wild fauna and new variety of _Trichinella britovi _identification | |
| 2009 Veterinary Parasitology (159): 222-224 | |
_Trichinella spiralis_ and _Trichinella britovi_ live in apparent sympatry among wild fauna of the Iberian Peninsula. In the present study 105 _Trichinella _isolates from wild mammals were typed by inter-sequence simple repeat PCR (ISSR-PCR). All isolates identified as _T. spiralis_ were indistinguishable from the ISS48 reference strain. Among those belonging to _T. britovi_, four variations were clearly distinguishable; two of them, ISS11 C-76 and ISS86 MON, had been previously detected while the ISS2 reference strain and Trichinella Rioja 3, (MVUL/SP/02/R3) had not been reported before. The newly distinguished genotype of _T. britovi_ was analyzed by ISSR-PCR,multiplex-PCR, UARR sequencing, and single larva crossbreeding with the other _T. britovi_ genotypes including _Trichinella_ T8 (ISS49). Among all of them, the ISS11 and ISS2 isolates were found to be the most frequent. The uniformity found within _T. spiralis_ isolates is consistent with its recent introduction in Iberian Peninsula, whereas the presence of four variations within _T. britovi _suggests that this species is an endemic species. Orographical diversity of the West-End of Eurasian Region could act to preserve population diversity observed within _T. britovi__._ |
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