IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Perteguer, M.J.; Rodr¡guez, E.; Garc¡a-S nchez, R.N.; Nogal-Ruiz, J.J.; Bolas-Fern ndez, F.; Mart¡nez-Fern ndez, A.R.; G rate, T. | |
| Identification of Spanish _Trichinella_ isolates by ISSR-PCR: Intra-specific variability of _Trichinella_ britovi | |
| 2009 Veterinary Parasitology (159): 206-209 | |
In the present work, we investigated genetic variability of the Spanish _Trichinella_ isolates by ISSR-PCR (inter-simple sequence repeat polymerase chain reaction), a technique that is being successfully used to study diversity among related populations.Werecovered a total of 43 isolates from different host and geographic localization and identified them by molecular techniques (RAPD and multiplex-PCR) and by Western blot with monoclonal antibodies US5 and US9. Nineteen (44.2%) out of 43 were identified as _Trichinella spiralis_ and 24 (55.8%) as _Trichinella britovi_. When these samples were analysed by the ISSR technique, all the _T. spiralis_ isolates presented a pattern similar to the _T. spiralis_ ISS116. By contrast, the ISSR-PCR analysis of the isolates identified as _T. britovi_, showed two different banding profiles compatible with the European _T. britovi _isolate pattern (ISS2), and the autochthonous Spanish _T. britovi_ isolate (ISS11). Three of these 43 isolateswere involved in human outbreaks; the three were identified as _T. britovi_ and showed a pattern similar to the European isolate ISS2. As conclusion, we highlight that an intra-species variability within the Spanish _T. britovi _isolates analysed was observed, with a predominant group similar to _T. britovi_ ISS2, while _T. spiralis_ group isolates were more homogeneous. No correlations were found between the different ISSR-PCR T. britovi types and the host/geographical origin of the isolates. |
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