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Wei, K.; Zhang, Z.; Zhang, W.; Xu, X.; Xu, L.; He, G.; Shen, F.; Zhang, L.; Hou, R.; Yue, B.
PCR-CTPP: a rapid and reliable genotyping technique based on ZFX/ZFY alleles for sex identification of tiger (_Panthera tigris_) and four other endangered felids
2008 Conservation Genetics (9): 225-228
An inexpensive, time-saving and reliable method, polymerase chain reaction with confronting two-pair primers (PCR-CTPP), was developed for sex identification in tiger (_Panthera tigris_) based on zinc finger alleles (ZFX/ZFY). A site of ''C/G'' transversion representing fixed differences that discriminated between ZFX and ZFY exons among felids was identified for primers designing. This primer set was successfully tested on samples including blood, shed hairs, dried skin, and stool which contained potential contamination caused by prey DNA. Cross species tests shown that this primer set was also useful for sex identification in four other endangered felids.
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