IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Rozhnov, V.V.; Sorokin, P.A.; Naidenko, S.V.; Lukarevskiy, V.S.; Hernandez-Blanco, H.A.; Litvinov, M.N.; Kotlyar, A.K.; Yudin, V.G. | |
| Noninvasive Individual Identification of the Amur Tiger (_Panthera tigris altaica_) by Molecular-Genetic Methods | |
| 2009 Doklady Biological Sciences (429): 518-522 | |
The procedure of noninvasive individual identification of tigers by moleculargenetic methods was developed within the framework of the Program of the Amur Tiger Research in the Russian Far East. Using this procedure, the number, sex, and relationships in a group of tigers in Ussuri Nature Reserve, Far East Branch, Russian Academy of Sciences was defined.To develop the procedures for noninvasive individual identification of the Amur tiger in the Far East of Russia, samples of blood, hair, and feces of animals were collected. The material for analysis was collected in the Zoological Center of the Institute of Biology and Soil Sciences, Far East Branch of the Russian Academy of Sciences (Gaivoron village, Spassk raion, Primorskii krai); in the Sysoev Priamurskii Zoo (Khabarovsk krai); and in the Komarov Ussuri Nature Reserve, Far East Branch of the Russian Academy of Sciences. As a result of genotyping of 35 samples of feces of the Amur tiger, collected on the territory of the Ussuri Nature Reserve during field research, we determined the number and sex of the animals that use this terri tory all year round. These were an adult female tiger, which was caught and tagged with a GPS Argos transmitter collar, and its three offspring, which carried one maternal allele in each microsatellite locus. In addition, we managed to genotype a male tiger, possibly the father of these three cubs, which was seen in the reserve in the period from the spring of 2008 to the spring of 2009. |
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