IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Crespo, C.; Gomendio, M.; Roldan, E.R.S. | |
| Cryobanking of Iberian Lynx (_Lynx pardinus_) Somatic Cells: Optimization of Isolation, Culture and Cryopreservation for a Genetic Resource Bank | |
| 2007 Conference Proceeding | |
Iberian lynx is critically endangered. Despite considerable efforts for conservation in its natural habitat, population size is decreasing. When an animal dies, its genotype is lost. This is particularly critical for immature individuals that never reproduce. To preserve a maximum of genetic diversity we set up schemes to routinely bank somatic tissues and cells that could be used in future research and in the development of nuclear transfer techniques that can benefit _in situ _and_ ex situ_ conservation programmes. Since 2004, tissue samples from 16 necropsied and from 55 biopsied individuals from Do¤ana and Sierra Morena were collected. Tissues were routinely cryopreserved in liquid nitrogen and cultured for fibroblast isolation and growth. Cells were successfully isolated, cultured and cryopreserved from 93% of necropsies and from 84% of biopsies (some cultures are ongoing). Cryopreserved tissues were found to grow in culture after months of storage. Factors affecting cell viability (e.g. time of recovery), isolation (explant or collagenase treatment), culture, and cryopreservation under various conditions were examined in detail in biomaterials obtained from necropsies. These studies reveal the feasibility of rescuing and banking biomaterials from dead and live animals from wild and captive populations. _Funded by Ministry of the Environment and CSIC._ |
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