IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Leon-Quinto, T.; Simon, M.A.; Sanchez, A.; Martin, F.; Soria, B. | |
| Cryobanking the genetic diversity in the critically endangered Iberian lynx (_Lynx 3 pardinus_) from skin biopsies. Investigating the cryopreservation and culture 4 ability of highly valuable explants and cells | |
| 2011 Cryobiology (62): 145-151 | |
Cryobanking skin samples permit preserving a maximum of genetic representation from the population biodiversity. This is a relevant aspect for threatened species, potentially menaced by an epizooty and from which it is difficult to obtain gametes. As a first step for properly cryobanking skin samples of agiven species, the optimal conditions of culture and freezing have to be studied by covering a broad range of possibilities. This paper presents, for the first time, a systematic study of such conditions for the Iberian lynx (Lynx pardinus). To that end, we have analyzed twenty different culture conditions and fifteen dif- ferent freezing solutions for skin explants, as well as three freezing solutions for isolated cells derived from them. The culture conditions included both two different culture strategies and several combina- tions of nutritional supplements and mitotic agents. For the freezing solutions, we have considered dif- ferent concentrations of the permeating cryoprotectant dimethyl sulfoxide (Me2SO) either alone (5%, 7.5%, 10%, 12.5% and 15% v/v for explants, 10% for isolated cells) or along with the non-permeating cryo- protectant sucrose (0.1 or 0.2 M). Our results have been analyzed through several quantitative parame- ters and show that only thawed explants cryopreserved in Me2SO (10%) either alone or with sucrose (0.2 M) presented similar properties to those in optimal fresh cultures. In addition, for these freezing con- ditions, isolated thawed cells also presented high survival rates (90%) and percentages of cellular func- tionality (85%). These results, focussed on the most endangered felid in the world, could be also useful for other threatened/endangered species. |
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