IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Kennedy, M.; Dolorico, T.; McNabb, A.; Moffatt, A.; Stylianides, E.; van Vuuren, M.; Kania, S. | |
| Characterization of Feline Coronavirus of cheetahs_ (Acinonyx jubatus) _- diagnosis, epidemiology, and viral genetic analysis | |
| 2000 Conference Proceeding | |
Cheetahs (Acinonyx jubatus) are susceptible to severe, often lethal disease following infection with Feline Coronavirus (FCV). We have investigated the molecular genetics of FCV of cheetahs focusing on the 7a7b genes which are believed to be associated with virulence. The methods utilized include RNA extraction, reverse transcription and nested polymerase chain reaction (PCR). Virus detection by PCR was compared to serology for FCV which is currently used for screening for infection. In addition, samples were collected from one institution at multiple time points over one year in order to evaluate the epidemiology of FCV in a captive population. The results thusfar show 1) that the antigenicity of the capture antigen significantly impacts results, 2) seronegative animals may be shedding virus while seropositive animals may not, 3) evidence of persistent infection, increased shedding during periods of stress, and direct and indirect transmission in captive populations, and 4) mutations occur in the 7a7b genes that may impact virulence. |
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