IUCN / SSC Cat Specialist Group - Digital Cat Library |
|
||||||||||
|
||||||||||
|
|
| Fitak, R.R.; Naidu, A.; Culver, M. | |
| PumaPlex: a rapid and high-throughput method to genotype single nucleotide polymorphisms in mountain lion tissues and scats | |
| 2014 Conference Proceeding | |
Population genetic analyses have become a powerful tool for the conservation and management of wildlife populations. However, the most common genetic markers, microsatellites, of ten incur a high cost of examination, suffer from homoplasy, and produce data difficult to compare across studies. Recently, alternative genetic markers, called single nucleotide polymorphism (SNPs), have received much attention for their analytical simplicity, high-throughput capabilities, consistency across studies, and potential utility in ancient, degraded, and non-invasively collected samples. In our study we designed a high-throughput assay (called PumaPlex) to simultaneously genotype 26 SNPs in mountain lions (_Puma concolor_). To date, we have genotyped more than 700 mountain lions, including ~500 individuals from Arizona. Our results from using PumaPlex produced comparable results to that of microsatellites, with a substantial decrease in overall costs and turnaround time. Additionally, we directly compared genotyping success of PumaPlex with a panel of 12 well-known _Felis catus_ (FCA) microsatellite loci in 46 mountain lion scat samples. We found that measures of genotyping success were significantly improved for PumaPlex relative to microsatellites. The advantages of PumaPlex compared with traditional microsatellite genotyping make it a valuable addition to existing methods to assist genetic monitoring and management of mountain lions throughout their range. |
|
 |
PDF files are only accessible to Friends of the Cat Group. Joining Friends of the Cat Group gives you unlimited access and downloads in the Cat SG Library for one year, and allows you to receive our newsletter Cat News (2 regular issues per year plus special issues). More information how to join here |
(c) IUCN/SSC Cat Specialist Group ( IUCN - The World Conservation Union) |